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Abstract #0448

Oxidative stress measured by in-vivo, longitudinal 1H MRS and ex-vivo ESR spectroscopy in a rat model of chronic Hepatic Encephalopathy

Katarzyna Pierzchala1, Veronika Rackayova1, Olivier Braissant2, Dario Sessa3, Stefanita Mitrea1, Andrzej Sienkiewicz4, Valérie A. McLin3, Rolf Gruetter1, and Cristina Cudalbu1

1Center for Biomedical Imaging, EPFL, Lausanne, Switzerland, 2Service of Biomedicine, CHUV, Lausanne, Switzerland, 3Swiss Center for Liver Disease in Children, Department of Pediatrics, HUG, Geneva, Switzerland, 4Laboratory of Physics of Complex Matter, EPFL, Lausanne, Switzerland

Oxidative stress is believed to play a role in the pathogenesis of chronic hepatic encephalopathy (CHE), however no in-vivo studies to assess the course of cerebral oxidative stress in CHE were performed. We investigated the oxidative stress in the hippocampus of a rat model of chronic HE using in-vivo and longitudinal 1H-MRS combined with ex-vivo ESR spectroscopy. Early changes in brain antioxidants (Asc, 4weeks post-BDL), concomitant with Gln and NH4+ changes were detected for the first time in-vivo. These results were confirmed by ESR spectroscopy suggesting that central oxidative stress is an early event in CHE.

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