Abstract #0447

Effects of Deuteration on Pyruvate Metabolism in the Isolated Heart

Alexander Max Funk¹, Nesmine Maptue¹, Chalermchai Khemtong¹, Dean Sherry^1,2, and Craig Malloy^1,3

¹UT Southwestern Medical Center, Dallas, TX, United States, ²University of Texas at Dallas, Richardson, TX, United States, ³Veterans Affairs North Texas Healthcare System, Dallas, TX, United States

Deuteration of metabolites is increasingly popular in hyperpolarization experiments as it prolongs the T₁ of the ¹³C resonances. However, the effect of deuteration on metabolism needs to be investigate to validate the hyperpolarization experiments. Kinetic isotope effects of deuteration of pyruvate were analyzed in isolated rat hearts. No kinetic isotope effect were observed in the pyruvate pool (lactate/alanine) or the TCA cycle (glutamate C5), but a ²H-¹H displacement was observed in alanine transaminase and an increase of the contribution of unlabeled sources.

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