Meeting Banner
Abstract #0447

Effects of Deuteration on Pyruvate Metabolism in the Isolated Heart

Alexander Max Funk1, Nesmine Maptue1, Chalermchai Khemtong1, Dean Sherry1,2, and Craig Malloy1,3

1UT Southwestern Medical Center, Dallas, TX, United States, 2University of Texas at Dallas, Richardson, TX, United States, 3Veterans Affairs North Texas Healthcare System, Dallas, TX, United States

Deuteration of metabolites is increasingly popular in hyperpolarization experiments as it prolongs the T1 of the 13C resonances. However, the effect of deuteration on metabolism needs to be investigate to validate the hyperpolarization experiments. Kinetic isotope effects of deuteration of pyruvate were analyzed in isolated rat hearts. No kinetic isotope effect were observed in the pyruvate pool (lactate/alanine) or the TCA cycle (glutamate C5), but a 2H-1H displacement was observed in alanine transaminase and an increase of the contribution of unlabeled sources.

This abstract and the presentation materials are available to members only; a login is required.

Join Here