In this study for the first time cerebral NAA, Asp and Glu concentrations of major inhibitory were simultaneously estimated in patients severe TBI . This work revealed significant reduction NAA and Asp concentrations in frontal lobe (on 65 and 61% respectively) in patients with severe TBI as compare to control group. At the same time, there was no significant change in Glu concentration. Our findings indicate that the main reason for the [NAA] reduction in the chronic severe TBI is caused by a decrease in [Asp]. Precursor of synthesis [NAA]. [Asp] reduction along with an unchanged Glu level may be caused by the dysfunction of one of the most important metabolic regulation systems - the malate-aspartate shuttle (MAS).