Meeting Banner
Abstract #0781

CEST MRI detection of Deoxycytidine Kinase activity using natural deoxycytidine

Zheng Han1, Jia Zhang1, Yuguo Li1, Jing Liu1, Peter van Zijl1, and Guanshu Liu1,2

1Radiology, Johns Hopkins University, Baltimore, MD, United States, 2F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, Baltimore, MD, United States

To assess the activity of deoxycytidine kinase (dCK), a drug-resistance-determining enzyme, we developed a new CEST MRI approach, in which the natural substrate, deoxycytidine (dC), is directly used as a CEST MRI reporter. Both in vitro and in vivo results showed that, upon the incubation or injection of dC, dCK(+) tumor cells have significantly higher CEST contrast at 2 ppm than dCK(-) cells, attributed to the accumulation of phosphorylated dC by dCK activity. Because dC is a clinically available agent, this ‘natural” MR molecular imaging method has great potential to be quickly translated to the clinic for patient stratification.

This abstract and the presentation materials are available to members only; a login is required.

Join Here