Meeting Banner
Abstract #0780

Hyperpolarized 13C β-hydroxybutyrate/acetoacetate as a biomarker for non-invasive monitoring of NAD+/NADH status in glioblastoma

Chloe Najac1, Marina Radoul1, Lydia M Le Page1,2, Georgios Batsios1, Pavithra Viswanath1, Anne Marie Gillespie1, and Sabrina M Ronen1

1Department of Radiology and Biomedical Imaging, University of California San Francisco, San Francisco, CA, United States, 2Department of Physical Therapy and Rehabilitation Science, University of California San Francisco, San Francisco, CA, United States

Conversion of acetoacetate (AcAc) to β-hydroxybutyrate (β-HB) by the mitochondrial enzyme β-hydroxybutyrate dehydrogenase depends upon NADH availability. Previous studies have shown the potential of β-HB-to-AcAc ratio to reflect the redox state in rat hearts and lymphoma cells. Here, we assessed the value of HP [1,3-13C]-AcAc in brain. We demonstrated the potential to probe AcAc to β-HB conversion in normal mice and mice with glioblastoma. Significantly higher levels of [1-13C]-β-AcAc and [1-13C]-β-HB were observed in tumor-bearing mice compared to control mice. Consistent with lower levels of NADH measured in tumors, the [1-13C]-β-HB-to-[1-13C]-β-AcAc ratio trended towards a decrease compared to normal brain.

This abstract and the presentation materials are available to members only; a login is required.

Join Here