Meeting Banner
Abstract #0806

In vivo monitoring of intracellular pO2 in response to CAR T cell immunotherapy against glioma

Fanny Chapelin1, Wenlian Zhu2, Hideho Okada3, and Eric Ahrens2

1Bioengineering, UCSD, La Jolla, CA, United States, 2Radiology, UCSD, La Jolla, CA, United States, 3Neurological Surgery, UCSF, San Francisco, CA, United States

We explore the temporal dynamics of tumor intracellular partial pressure of oxygen (pO2) in a murine glioma model receiving human chimeric antigen receptor (CAR) T cell immunotherapy. Tumor cells were intracellularly labeled with perfluoro-crown-ether (PCE) nanoemulsion ex vivo before flank injection in mouse. The spin-lattice relaxation rate (R1) of PCE is linearly proportional to the local oxygen concentration, enabling longitudinal in vivo measurement of absolute pO2 values. Our results indicate that CAR T cell therapy induces significant tumor pO2 increase peaking three days post-intravenous injection and correlates to tumor growth reduction.

This abstract and the presentation materials are available to members only; a login is required.

Join Here