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Abstract #0923

Correlations between quantiative myelin imaging using macromolecular proton fraction, neurogenesis, and oligodendrogenesis in the murine model of cuprizone-induced demyelination

Marina Khodanovich1, Anna Pishchelko1, Valentina Glazacheva1, Edgar Pan1, Andrey Akulov1,2, and Vasily Yarnykh1,3

1Research Institute of Biology and Biophysics, Laboratory of Neurobiology, Tomsk State University, Tomsk, Russian Federation, 2Institute of Cytology and Genetics SB RAS, Novosibirsk, Russian Federation, 3Dapartment of Radiology, University of Washington, Seattle, WA, United States

This study aimed to identify associations between neurogenesis in the neurogenic niches, oligodendrogenesis, and myelination in the murine cuprizone demyelination/remyelination model. Myelination was quantified by the recently proposed macromolecular proton fraction (MPF) mapping method. Neurogenesis and oligodendrogenesis were assessed by immunohistology. Negative correlations were found between oligodendrogenesis and neurogenesis in both the subventricular zone and dentate gyrus. Correlation between MPF and oligodendrogenesis was also negative, whereas correlation between MPF and neurogenesis was positive. Associations between MPF and neurogenesis/oligodendrogenesis reveal the feasibility of using MPF as a surrogate marker of reparative processes in demyelinating diseases.

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