Deuterium Metabolic Imaging (DMI) is a novel approach providing high spatial resolution metabolic data from both animal models and human subjects. DMI relies on 2H MRSI in combination with administration of 2H-labeled substrates. We show how DMI combined with administration of [6,6’-2H2]-glucose can image liver glycogen synthesis in rats and human subjects. In rats at 11.7T, DMI revealed differences in liver glycogen synthesis between glucose administrations through an intravenous and intraperitoneal route. At 4T, we showed that DMI is feasible in humans and could detect labeling of liver glycogen after oral intake of [6,6’-2H2]-glucose.