In this study, double diffusion encoding (DDE) MRI measurements of microscopic fractional anisotropy (μFA) are compared at two different echo times (TE). At longer TE, higher mean μFA values were measured in single fiber regions but not in crossing fiber regions. Consistent with prior work on the TE dependence of fractional anisotropy (FA) the largest differences were found in regions known to be highly myelinated such as the genu of the corpus callosum.
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