To evaluate the value of diffusional kurtosis imaging (DKI) in renal fibrosis using a rat model of unilateral ureteral obstruction (UUO). Differences of DKI parameters among the time points and between the sides were compared. The correlation of DKI parameters with positron emission tomography (PET) renal function and expression of the fibrosis maker α-SMA were determined. There were significant differences on mean diffusivity (MD) and fractional anisotropy (FA) among days and between two sides. FA was moderate correlated with SUV and α-SMA. Therefore, quantitative DKI could be considered a useful and noninvasive method to help assess renal fibrosis.