Metabolic profiling of tissue biopsies using HR-MAS NMR has potential diagnostic and prognostic value, but alterations in the biochemical profile due to factors such as sampling method may lead to misinterpretation. Therefore we investigated the effect of two different sampling methods in normal caprine brain tissue, in vivo sampling by stereotactic biopsy and direct post mortem surgical sampling. We found significant differences between the two biopsy types with elevated lactate and creatine, and altered choline-containing compounds. We conclude that metabolite alterations depend on sampling methods and suggest the use of in vivo biopsy in animal models.
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