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Abstract #1852

Effect of aldehyde fixation on the myelin water fraction measurements in rat cervical spinal cord

Henry Szu-Meng Chen1, Jie Liu2, Alex L. MacKay1,3,4, Wolfram Tetzlaff2,5, and Piotr Kozlowski1,4

1Radiology, University of British Columbia, Vancouver, BC, Canada, 2ICORD, Vancouver, BC, Canada, 3Physics and Astronomy, University of British Columbia, Vancouver, BC, Canada, 4UBC MRI Research Centre, Vancouver, BC, Canada, 5Zoology, University of British Columbia, Vancouver, BC, Canada

This study investigated the effect of tissue fixation on myelin water fraction (MWF), an MR derived measurement of myelin content. MWF was found to increase during aldehyde fixation due to an increase in myelin water. Differences in MWF between immersion fixation and perfusion fixation with immersion post-fixation were quantified. This study demonstrated that the measured MWF is sensitive to the changes induced by chemical fixation. The results bridge the interpretation of MWF in the in vivo situation to that of the ex vivo situation and provide a guideline for designing MWF studies with histological validation.

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