Neurometabolic rate is coupled with neurotransmitter cycling, which is perturbed during various neurological disorders. Though, anesthetics are widely used in neurometabolic studies, their impacts on neural function is unclear. In the present study, effects of isoflurane and urethane on brain energy metabolism were investigated using 1H-[13C]-NMR spectroscopy in tissue extract during an infusion of [1,6-13C2]glucose or [2-13C]acetate. The reduction in neuronal metabolic activity under isoflurane was higher than urethane in the cerebral cortex and striatum. The data from the study indicate that impacts of anesthetics on neuronal function is more compared to astroglia suggesting that astroglial function is less affected with increased brain activity.