Depression is a common and serious medical illness with a direct impact both in the physical and mental health. It is a complex disorder of the mood with a high incidence in the world population and a tendency to continue increasing. The multifactorial and heterogeneous character of this disease hinders the understanding of the pathological mechanism. The use of an appropriate animal model of depression can contribute to improve the diagnosis and monitoring of the therapy outcome. In this work, we characterized with MRI and phenotyping studies a mild depression model developed in female rats.