Nowadays, the assessment of brain functional connectivity (FC) patterns, ranging from resting-state networks to network modelling, can rely on Arterial Spin Labeling (ASL) MRI as an alternative to the gold-standard sequence represented by the blood-oxygenation-level-dependent contrast. We evaluated FC mapping from different perspectives (experimental protocols, populations and analysis methods), trying to overcome some of the present challenges related to the ASL applicability in this framework. The results demonstrate how FC patterns and changes can be reliably detected using ASL, with the added value of allowing the simultaneous quantification of brain perfusion, a direct marker of neuronal activity.
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