Diffusion Tensor Imaging (DTI) is a reliable tool for investigating renal microstructure and renal function, the imaging stability remains challenging. Recently, the image-quality improvement by zoom DTI technique (reduced Field-Of-View diffusion) is reported1. We scanned 10 healthy volunteers by this technique via the respiration-triggered acquisition, and we assessed different ROIs within the medulla and the cortex of the kidney. In this study, the reproducibility between different subjects in zoom DTI was more promising when compared to full-FOV DTI. More DTI scalars were compared between zoom and full-FOV DTI in cortex and medulla and these may be potential parameters to detect pathological changes in kidney.