Chronic mesenteric ischemia (CMI) is caused by inadequate blood flow to the intestines. This study investigates the use of 4D flow MRI to non-invasively assess the hemodynamics of the mesenteric circulation in patients with CMI and controls. Flow was measured in 9 vessels before and after meal challenges for 19 subjects suspected of CMI and 6 controls. Post-prandial flow increased significantly in the supraceliac aorta, superior mesenteric artery, superior mesenteric vein, and portal vein. The flow increase was drastically stunted in patients with CMI. This demonstrates the potential for 4D flow MRI in assisting the challenging diagnosis of CMI.