Advanced renal disease is characterized by adverse changes in renal structure, however non-invasive diagnostic imaging techniques are currently lacking. Here we describe the assessment and reproducibility of native T1 mapping for renal tissue characterization. Renal native T1 mapping was performed in 15 healthy human volunteers using the Modified Look-Locker Imaging (MOLLI) 5s(3s)3s sequence on a clinical 3.0 T MR system. Found intra- and inter-examination ICCs for renal cortex (0.77, 0.65) and medulla (0.65, 0.99) indicate good intra- and inter-examination reproducibility, combined with the Bland-Altman analysis showing good agreement. Renal native T1-mapping is a promising reproducible technique for renal tissue characterization.