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Abstract #3040

Manganese enhanced MRI in organotypic rat hippocampus slices: A correlative study with synchrotron X-ray nanoprobe analysis and electron microscopy.

Alexia Daoust1, Natalia B Pivovarova1, Emily Petrus1, S Brian Andrews1, Barry Lai2, Si Chen2, Maria A Aronova3, Richard D Leapman3, and Alan P Koretsky1

1National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, United States, 2Argonne National Laboratory, Advanced Photon Source, Argonne, IL, United States, 3National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, United States

Manganese (Mn2+) Enhanced Magnetic Resonance Imaging (MEMRI) can be used for different applications such as tracing neuronal connections or functional imaging. However, Mn2+ uptake and transport mechanisms are still unclear. These mechanisms were studied by imaging sub-cellular Mn2+ in an organotypic hippocampal slice culture by coupling MEMRI, TEM and X-ray methodologies. The data indicates that Mn2+ is located at synapses but not in mitochondria.

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