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Abstract #3046

A proof-of-concept study on the quantification of gene expression levels with doxycycline-inducible MR reporter gene

Seul-I Lee1, Jeeheon Kang1, Yoonseok Choi2, Jinil Kim 1, Jae-Im Kwon1, Ho-jin Kim1, Su Jung Ham3, Sang-Tae Kim1, Chul Woong Woo1, Do-Wan Lee1, Dong-Cheol Woo1, and Kyung Won Kim3

1MR Core, Asan medical Center, Seoul, Republic of Korea, 2GangNeung Asan Medical Center, GangNeung, Republic of Korea, 3Radiology, Asan medical Center, Seoul, Republic of Korea

Recent research on MR reporter genes has demonstrated their potential for use in transgene expression monitoring. We have conducted a preliminary study on the development of a new MRI reporter system [organic anion transporting polypeptide (OATP) 1B1] that can analyze gene expression level using MR reporter genes. By establishing doxycycline-inducible cell line, we observed T1 shortening on MRI, which indicated increased expression level of the OATP1B1 gene. A strong correlation was observed between conventional methods for measurement of gene expression and rrT1 of MR imaging. In this study, we provide preliminary evidence of the potential application of MRI to determine gene expression.

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