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Abstract #3049

Magnetic Resonance Tracking of Iron-Labeled Stem Cells After Osteochondral Defect in Ovine Model

Joshua Kaggie1,2, Martin J Graves1,2, James MacKay1,2, Scott Reid3, Hareklea Markides4, Alicia El Haj4, Stephen McDonnell2,5, Fiona J Gilbert1,2, Andrew McCaskie2,5, and Frances Henson6

1Radiology, University of Cambridge, Cambridge, United Kingdom, 2Addenbrooke's Hospital, Cambridge University Hospitals NHS Foundation Trust, Cambridge, United Kingdom, 3GE Healthcare, Amersham, United Kingdom, 4Institute of Science and Technology in Medicine, Keele University, Newcastle, United Kingdom, 5Division of Trauma and Orthopaedic Surgery, University of Cambridge, Cambridge, United Kingdom, 6Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom

Multipotent mesenchymal stem cells (MSCs) can be labeled with superparamagnetic iron-oxide nanoparticles (SPION) particles to track single cells with MRI, and thereby follow MSC infiltration. However, a limitation with conventional MR sequences is that their long echo times are unable to measure fast signal decays, which occur in dense bone tissue and with high SPION infiltration. Ultra-short echo time (UTE) MRI can capture these rapidly decaying signals. In this work, we use 3D cones to track tissue development after injection of SPION labeled MSCs in an ovine model.

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