Abstract #3048

An Improved CEST MRI Reporter Gene for Molecular Imaging of Cell and Viral Based Therapeutics

Christian T. Farrar¹, Hirotaka Ito², Hiroshi Nakashima², E. Antonio Chiocca², and Assaf A. Gilad^3,4,5

¹Martinos Center for Biomedcial Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA, United States, ²Department of Neurosurgery, Brigham & Women's Hospital and Harvard Medical School, Boston, MA, United States, ³Department of Biomedical Engineering, Michigan State University, East Lansing, MI, United States, ⁴The Institute of Quantitative Health Science and Engineering, Michigan State University, East Lansing, MI, United States, ⁵Department of Radiology, Michigan State University, East Lansing, MI, United States

The ability to image cell- or viral-based therapeutics is critical for optimizing therapeutic strategies and assessing efficacy. A lysine rich protein (LRP) chemical exchange saturation transfer (CEST) MRI reporter gene has previously been developed and successfully used to image oncolytic viruses and tumor cells. However, the highly repetitive nature of the LRP reporter gene sequence lead to DNA recombination events and the expression of a range of truncated LRP protein fragments, thereby greatly limiting the CEST sensitivity. Here we report the use of a redesigned LRP reporter (rdLRP), which demonstrated excellent stability and CEST sensitivity.

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