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Abstract #3066

Assessing γ-glutamyl transpeptidase activity in kidney using hyperpolarized γ-Glu-[1-13C]Gly

Steffen F. Frank1, Hikari A. I. Yoshihara1, Marino Itoda2, Shinsuke Sando2, and Rolf Gruetter3,4,5

1Laboratory for Functional and Metabolic Imaging, EPFL, Lausanne, Switzerland, 2Department of Chemistry and Biotechnology, The University of Tokyo, Tokyo, Japan, 3Department of Radiology, University of Geneva, Geneva, Switzerland, 4Department of Radiology, University of Lausanne, Lausanne, Switzerland, 5Centre for Biomedical Imaging, EPFL, Lausanne, Switzerland

Hyperpolarized γ-Glu-[1-13C]Gly provides a non-invasive means to detect γ-glutamyl transpeptidase (GGT) enzyme activity in vivo and indicates its potential for application in functional imaging. Since GGT is most abundant in the proximal tubules of the kidney, and since the properties of γ-Glu-[1-13C]Gly are suitable for in vivo hyperpolarized 13C metabolic analysis, it was proposed as a molecular probe to study kidney function. The aim of the present study is to identify the dose of γ-Glu-[1-13C]Gly that gives high NMR sensitivity in the unsaturated state of the GGT enzyme.

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