Abstract #3069

Monitoring effect of rapamycin on pyruvate metabolism in SCC tumor using hyperpolarized 13C-MRI

Keita Saito¹, Shingo Matsumoto^1,2, Yoichi Takakusagi^1,3, Masayuki Matsuo^1,4, Hellmut Merkle⁵, James B Mitchell¹, and Murali C Krishna¹

¹National Cancer Institute, Bethesda, MD, United States, ²Hokkaido University School of Engineering, Sapporo, Japan, ³National Institutes for Quantum and Radiological Science and Technology, Chiba, Japan, ⁴Gifu University School of Medicine, Gifu, Japan, ⁵National Institute of Neurological Disorder and Stroke, Bethesda, MD, United States

Effect of an mTOR inhibitor rapamycin on pyruvate metabolism in squamous cell carcinoma (SCC) xenografts was investigated using hyperpolarized ¹³C-MRI. [1-¹³C]lactate to [1-¹³C]pyruvate ratio (Lac/Pyr) in the SCC tumors increased as tumor grew in non-treated control mice, whereas it significantly dropped after 2 days of the rapamycin treatment. Inhibition of mTOR caused a drop of LDH protein level and the activity in the SCC tumor, and perfusion in the tumor was improved by the rapamycin treatment. Lac/Pyr monitored using hyperpolarized ¹³C-MRI would become a useful marker for tumor response to mTOR inhibitors.

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