We investigated potential structural changes in peripheral nerves following ankle sprain injuries. Specifically, we assessed the integrity of the sciatic nerve and its major divisions: the tibial and peroneal nerves. Reduced field-of-view DTI was used, and the reproducibility of the DTI measures was examined. Our results revealed excellent reliability of DTI measures in injured versus control nerves across each parameter (FA, AD, RD, MD). A comparison of injured versus control nerves in the associated nerve branch indicated significant difference in AD. Given these results, DTI may have potential as a powerful tool to determine disease profile.