Diffusion parameters such as diffusivity, compartment fractions and diffusion signal itself are informative bio-markers in understanding and analyzing pathological changes in biological tissue. One frequently needs to compute a representative value of a diffusion parameter in a homogeneous ROI. Here we compare and contrast 3 common choices that researcher would make in the course ROI analysis. Though ROI analysis is deemed to be a common practice, the critical choices of computational methods (depending on noise distribution and underlying model) can considerably affect its findings and conclusions.
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