Abstract #3240

Optimal tissue preparation for ex vivo preclinical imaging

Rachel L. C. Barrett^1,2, Diana Cash¹, Camilla Simmons¹, Tobias Wood¹, Anthony C. Vernon^3,4, Marco Catani^1,2, and Flavio Dell'Acqua²

¹Department of Neuroimaging, IOPPN, King's College London, London, United Kingdom, ²Natbrainlab, Department of Forensic and Neurodevelopmental Sciences, IOPPN, King's College London, London, United Kingdom, ³Department of Basic and Clinical Neuroscience, IOPPN, King's College London, London, United Kingdom, ⁴MRC Centre for Neurodevelopmental Disorders, King's College London, London, United Kingdom

Ex vivo imaging is beneficial for studying rodent brain microstructure in healthy and pathological tissue at high resolution. There are challenges however associated with changes in tissue properties resulting from fixation. We present a tissue preparation protocol optimised for diffusion MRI in the rat brain by varying fixative concentration, gadolinium concentration and rehydration time. By altering T1 and T2 relaxivity, we show how these factors can be combined to maximise SNR efficiency. Improving SNR efficiency in ex vivo diffusion MRI will allow higher spatial and angular resolution for studying tissue microstructure in the rodent brain.

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