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Abstract #3595

In Vivo Murine Cardiac 19F MRI and Tracking of PFCE- and FuGENE-labeled Progenitor Stem Cells in the C57BL/6 Mouse

Chris Constantinides1, Ricardo Carnicer Hijazo1, Andrew Shaw1, Jyoti Patel1, Edyta Swider2, Mangala Srinivas2, and Carolyn Carr1

1U. Oxford, Oxford, United Kingdom, 2Radboud University Medical Center, Nijmegen, Netherlands

Despite advances in the visualization of pre-labeled SCs with perfluorocarbon-ether-nanoparticles (PFCE-NPs) or other MRI contrast agents using 19F MRI, there have been no prior reports on murine cardiac 19F imaging of exogenously administered cardiac SCs following direct, intra-cardiac injections. To-this-date, human or murine in vivo cardiac studies have been limited, while prior reported imaging attempts in rats have been prohibitively lengthy, costly (use of multiple millions of cells/animal), and practically complex to reproduce in mice. We report herein significant enhancements of PFCE-NP label following labeling with the highly efficient agent FuGENE, thereby allowing in vivo murine cardiac 19F MRI visualization with minimal cellular toxicity.

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