Currently, only invasive methods exist to study the molecular effects of traumatic brain injury (TBI). By instead using magnetic resonance spectroscopy (MRS), a non-invasive, quantitative method can be used to safely assess the severity and location of injury. Acrolein, a biomarker of TBI, increases in rat brain tissue following TBI. Acrolein shows signature peaks downfield of water at 6.5 and 9.4 ppm. We have successfully measured whole-brain phantom-injected acrolein using Bruker 7T MRS sequences and determined T1 for acrolein, using NMR, so MRS parameters can be adjusted to maximize acrolein signal. Quantifying acrolein with MRS could provide a viable method to assess injury location and severity.