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Abstract #4010

In vitro screening of drug response in HIV-1 pseudovirus infected cells using NMR

Aruna Singh1, Muzamil Makhdoomi2, Kalpana Luthra2, Thirumurthy Velpandian3, and Rama Jayasundar1

1Department of NMR, All India Institute of Medical Sciences, New Delhi, India, 2Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, India, 3Department of Ocular Pharmacology & Pharmacy, All India Institute of Medical Sciences, New Delhi, India

There is a necessity for novel and quick drug response screening methods for providing lead information for drug discovery. In this context, NMR can be a potential screening method. This study has used proton NMR spectroscopy for drug response in HIV-1 psuedovirus infected TZM-bl cells after treatment with TAK 779 (positive control), and benzene extracts of Terminalia bellerica and Zingiber officinale. Significant reduction in metabolites was observed in infected cells and metabolites were subsequently restored closer to normal levels on treatment with positive control and plant extracts. The study has demonstrated NMR to be a quick screening method for drug response.

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