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Abstract #4025

Exploring Glutamate and Glutamine Metabolism Across Breast Cancer Subtypes using High-resolution Proton MRS Combined with Molecular Approaches

Caitlin M Tressler1, Vinay Ayyappan2, and Kristine Glunde2

1Russell H. Morgan Department of Radiology, Johns Hopkins University School of Medicine, Baltimore, MD, United States, 2Johns Hopkins University School of Medicine, Baltimore, MD, United States

We have studied glutamine metabolism in a panel of breast cancer cell types to examine the utilization of glutamine as an energy source in different subtypes of breast cancer. We utilized high-resolution 1H MRS detection of glutamine and glutamate from cell extracts to determine up regulation of glutamine metabolism as a whole followed by quantitative RT-PCR to begin to establish the critical pathways involved in altered glutamine metabolism within malignant cell types. Altered glutamine metabolite signatures may be used to identify malignancy within cells and key enzymes in glutamine metabolism may prove to be potential therapeutic targets.

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