This study presents utility of MRF derived relaxometry, and ADC mapping for differentiating transition zone prostate cancers from non-cancerous lesions. Based on targeted biopsy correlation, T1, T2, ADC were compared between cancer, prostatitis and normal transition zone (NTZ). Mean T1, T2 and ADC were significantly different between cancer and NTZ. Mean T1 and ADC were significantly different between prostate cancer and prostatitis. While ADC had an AUC of 0.821 for differentiating cancer and prostatitis, a combination of T1 and T2 mapping had an AUC of 0.875. Thus MRF can add significant value to ADC mapping in characterization of TZ lesions.