Over-treatment of prostate cancer is a significant problem in men’s healthcare. Development of non-invasive imaging tools for improved identification of prostate lesions can reduce over-treatment. We have built custom sodium MRI hardware to image and quantify tissue sodium concentration (TSC) in the human prostate. Sodium and multi-parametric MR images are co-registered to Gleason-graded post-prostatectomy histology, the current gold standard for prostate cancer lesion characterization. Our data shows a statistically significant, positive correlation of TSC with Gleason score. These data suggest that TSC measured by sodium MRI, in addition to multi-parametric MRI has utility for non-invasive characterization of prostate cancer.