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Abstract #4599

Analysis of kidney function and therapy monitoring with DCE-MRI in a murine model of septic shock

Claudia Weidensteiner1, Wilfried Reichardt1, Joachim Struck2, Anne Kirchherr3, Katja Wagner4, Florian Wagner4, and Dominik von Elverfeldt1

1Dept. of Radiology, Medical Physics, Medical Center University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany, 2AdrenoMed AG, Hennigsdorf, Germany, 3nanoPET Pharma GmbH, Berlin, Germany, 4Institute of Anesthesiological Pathophysiology and Process Engineering, University Hospital Ulm, Ulm, Germany

DCE-MRI with a 3D spoiled gradient echo sequence and injection of Gd-DTPA was used to analyze the kidney function in mice during septic shock (cecal ligation and puncture CLP model). Gd concentration time courses were analyzed without pharmacokinetic modelling. In renal cortex and medulla of septic mice a slower exponential decay compared to baseline or even non-exponential curve shapes were observed. In most septic mice there was no Gd accumulation in the urine in the bladder. Treatment with an antibody targeting adrenomedullin (a vasodilatory peptide) resulted in a faster half-life of tracer elimination in the kidneys compared to vehicle-treatment.

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