Amyloidosis is a multisystemic disorder frequently affecting the heart and causing heart failure. In this work, MR imaging and spectroscopy was implemented and applied to characterize myocardial structure and function as well as changes in fatty acid storage of the heart. We found that myocardial triglyceride-to-water ratio was significantly decreased in amyloidosis compared to age-and body mass index-matched controls. Myocardial triglyceride-to-water ratio showed a negative tendency with increasing markers of heart failure. It is concluded that proton spectroscopy may provide an additional biomarker to gauge progression of cardiac amyloidosis.