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Abstract #5190

New Tissue Saturation Method for the Detection of Therapeutic Cells in a Knee Joint

Djaudat Idiyatullin1, Michael Garwood1, and Sergey Magnitsky2

1Center for Magnetic Resonance Research and Department of Radiology, University of Minnesota Medical School, Minneapolis, MN, United States, 2Radiology, UCSF, San Francisco, CA, United States

Synapsis. Mesenchymal Stem Cells (MSCs) have a high potential for a treatment of bone diseases. We develop new acquisition protocol for the detection of therapeutic cells in knee joints. Labeling MSCs with iron oxide particles not only reduce T2* but also induce the resonance frequency shift of labeled cells. This shift enabled us to implement tissue saturation scheme and detect distinct hyperintense signal from grafted cells. New protocol allowed us to detect and quantify therapeutic cells for six days after implantation which was not possible before. Proposed protocol opens new opportunities for in vivo monitoring of cell therapy of bone disorders.

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