Track-Weighted Imaging was recently introduced as a qualitative tractography-based method with super-resolution properties and high anatomical contrast. We report the use of this method to explore the human retina. In healthy volunteers, we showed that reconstructions of neuroretinal fascicles were influenced by diffusion acquisition parameters, ocular laterality, and ocular dominance. We have raised the hypothesis that patients with anterior ischaemic optic neuropathy, a disease that leads to degeneration of neuroretinal cells, could present with specific neuroretinal fascicle injuries. We demonstrated that presence of the temporal fascicle in the affected eye provides an objective outcome radiological sign correlated with visual recovery.