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Abstract #5579

Awake rat functional magnetic resonance imaging using 3D printed restrainment kit and standard RF-coils

Petteri Stenroos1, Jaakko Paasonen1, Raimo Salo1, Kimmo Jokivarsi1, and Olli Gröhn1

1Biomedical Imaging Unit, A.I. Virtanen Institute for Molecular Sciences, Kuopio, Finland

Anesthetics are typically used in preclinical functional magnetic resonance imaging (fMRI) experiments to prevent animal movement and stress, but anesthetics can significantly affect the brain function. Therefore, several awake fMRI protocols have been developed, usually implementing house-made coils and holders. In present study, we developed a 3D printable restrainment kit that is compatible with standard Bruker rat coils and holders. Subsequently, resting state functional connectivity (RSFC) data was obtained under awake, lightly sedated, and anesthetized conditions. Our results show that even subanesthetic dose of isoflurane modulates FC significantly.

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