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Abstract #0204

Multimodal awake mouse imaging: from two-photon microscopy to BOLD-fMRI

Michèle Desjardins1,2, Kılıç Kıvılcım2,3, Martin Thunemann2, Céline Mateo2, Dominic Holland2, Christopher G. L. Ferri2, Jonathan Cremonesi2, Boaqiang Li4, Qun Cheng2, Kimberly L Weldy2, Payam A Saisan2, David Kleinfeld2, Takaki Komiyama2, Thomas T Liu2, Robert Bussell2, Eric C Wong2, Miriam Scadeng2, Andrew K Dunn5, David A Boas3, Sava Sakadžić4, Joseph B Mandeville4, Richard B Buxton2, Anders M Dale2, and Anna Devor2

1Physique, génie physique et optique, Université Laval, Québec, QC, Canada, 2University of California San Diego, La Jolla, CA, United States, 3Boston University, Boston, MA, United States, 4Martinos Center for Biomedical Imaging, MGH, Harvard Medical School, Charlestown, MA, United States, 5University of Texas at Austin, Austin, TX, United States

Functional Magnetic Resonance Imaging (fMRI) in awake behaving mice is well positioned to bridge the detailed cellular-level view of brain activity, which has become available due to recent advances in microscopic optical imaging and genetics, to the macroscopic scale of human noninvasive observables. Here, we demonstrate Blood Oxygen Level Dependent (BOLD) fMRI in awake mice implanted with chronic transparent cranial ''windows'', compatible with two-photon microscopy, optical imaging, and optogenetic light stimulation. We thus provide a proof of feasibility for multimodal imaging approaches in awake mice, which in the future can be extended to behavioral studies and biomedical applications.

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