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Abstract #0483

Activity of the Pentose Phosphate Pathway Is Increased in Hepatoma Using a Novel Tracer, [2,3-13C2]glucose

Min Hee Lee1, Craig Malloy1,2, Ian Corbin1, JunJie Li1, and Eunsook Jin1

1Advanced Imaging Research Center, UT Southwestern Medical Center, Dallas, TX, United States, 2Radiology and Internal Medicine, UT Southwestern Medical Center, Dallas, TX, United States

The pentose phosphate pathway (PPP) was investigated in a rat model of hepatoma and results were compared to normal liver and other tissues. A novel and specific tracer of the PPP, [2,3-13C2]glucose, is introduced. The resulting isotopomers are informative because [1,2-13C2]lactate arises only from glycolysis and [2,3-13C2]lactate arises only from the PPP. The PPP was more active in the fed vs. fasted state in most tissues. These results correlated with mRNA expression of key enzymes in the PPP, and flux through both the PPP and glycolysis was substantially increased in hepatoma compared to healthy liver.

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