White matter injury is common in neonates. The most common punctate white matter lesions (PWML) can disappear along with time and are easily missed diagnosis, but quantitative measurements may find more subtle alterations. Since DTI is not sensitive to detect the white-matter microstructural changes in mild PWML, alterations in gray-matter (GM) may provide additional knowledge for predicting prognosis. This study aims to quantitatively assess alterations of GM in neonates with mild PWML. Compared with controls, a significant reduction of cortical volume is observed in neonates with mild PWML, and cortical thickness is a sensitive biomarker for characterizing the GM abnormalities.