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Abstract #0647

Validating axonal directionality with 3D X-ray scattering

Marios Georgiadis1,2, Dmitry S. Novikov1, Zirui Gao2,3, Manuel Guizar-Sicairos3, Lin Yang4, Shirish Chodankar4, Jelle Veraart1, Ben Ades-Aron1, Choong Heon Lee1, Sunglyoung Kim1, Piotr Walczak5, Jiangyang Zhang1, Aileen Schroeter2, Markus Rudin2, and Els Fieremans1

1NYU School of Medicine, New York, NY, United States, 2ETH Zurich, Zurich, Switzerland, 3Paul Scherrer Institute, Villigen PSI, Switzerland, 4Brookhaven National Laboratory, Upton, NY, United States, 5Johns Hopkins Medicine, Baltimore, MD, United States

Diffusion MRI (dMRI) is sensitive to neuronal alignment, yet its directional signal does not depend only on the fiber orientation distribution function (fODF). Current validation methods for measuring and modeling the ODF have major limitations. Small-angle X-ray scattering (SAXS) produces directly structural signal, specific to myelin’s repetitive structural arrangement. We apply 3D scanning SAXS on mouse brain sections, retrieve fODFs and compare against dMRI-derived axonal directionality. We also apply SAXS-tensor tomography to mouse spinal cord, produce tractography maps and correlate dMRI- and SAXS-TT-derived fODF parameters. These demonstrate SAXS’s potential for providing novel microstructural insights and structurally validating dMRI-derived fODFs.

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