Meeting Banner
Abstract #0717

Probing the metabolic response to acute renal injury in mice using multiple hyperpolarized 13C substrates.

Camille Ansermet1, Gabriel Centeno1, Mario Lepore2, Stefanita Mitrea2, Analina da Silva2, Shinsuke Sando3, Dmitri Firsov1, and Hikari A. I. Yoshihara4

1Department of Pharmacology and Toxicology, University of Lausanne, Lausanne, Switzerland, 2Centre d'imagerie biomédicale (CIBM), Ecole polytechnique fédérale de Lausanne (EPFL), Lausanne, Switzerland, 3Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan, 4Laboratory for Functional and Metabolic Imaging (LIFMET), Ecole polytechnique fédérale de Lausanne (EPFL), Lausanne, Switzerland

Arginase-2 is upregulated following renal injury. This study used hyperpolarized pyruvate, arginine, citrulline and γ-glutamylglycine to probe changes in renal metabolism following ischemia-reperfusion in mice deficient in renal arginase-2. The kidney is a major site of arginine synthesis, and conversion of hyperpolarized citrulline to arginine can be detected, as can a signal consistent with argininosuccinate. While metabolic differences in mice lacking arginase-2 compared to controls are not readily apparent by this method, ischemia has a clear effect on several metabolite signals. Of the pyruvate metabolites, conversion to aspartate is significantly diminished, as is arginine and argininosuccinate production from citrulline.

How to access this content:

For one year after publication, abstracts and videos are only open to registrants of this annual meeting. Registrants should use their existing login information. Non-registrant access can be purchased via the ISMRM E-Library.

After one year, current ISMRM & ISMRT members get free access to both the abstracts and videos. Non-members and non-registrants must purchase access via the ISMRM E-Library.

After two years, the meeting proceedings (abstracts) are opened to the public and require no login information. Videos remain behind password for access by members, registrants and E-Library customers.

Click here for more information on becoming a member.

Keywords