Abstract #0822

Multimodality OATP1B3-enhanced reporter gene imaging of engineered cells via fluorescence, photoacoustic, and magnetic resonance imaging

Nivin N Nyström^1,2, Lawrence Yip^2,3, Jeffrey Carson^2,3, Timothy J Scholl^1,2, and John A Ronald^1,2

¹Medical Imaging Laboratories, Robarts Research Institute, London, ON, Canada, ²Medical Biophysics, University of Western Ontario, London, ON, Canada, ³Lawson Health Research Institute, London, ON, Canada

Reporter genes can generate information on cell fate in vivo. Multimodality imaging can mitigate limitations of individual imaging modalities; thus, multimodality reporters are highly sought. We present a trimodality reporter gene system based on human Organic Anion-Transporting Polypeptide 1b3 (Oatp1b3) and its ability to take up indocyanine green (ICG) for fluorescence and photoacoustic imaging, as well as gadolinium ethoxy benzyl diethylenetriamine pentaacetic acid (Gd-EOB-DTPA) for MRI. This technology should find utility in tracking cell- and gene-based therapies for preclinical studies, and due to the human-derivation of the reporter gene and already clinically-utilized probes, presents with high translational potential.

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