Although 3.0T MRI has been increasingly used for neonates, the strong noise remains a great concern. This study aimed to estimate the effect of MRI noise on neonatal cochlear function. Thirty ears of 17 patients with no hearing impairment were enrolled. Distortion product OAE (DPOAE) tests were performed to estimate the cochlear function. Significant increase of mean DPOAE amplitude by 1.13 dB at 3kHz was found within 30 minutes after MRI. Besides, standard deviations of DPOAE amplitude differences remarkably increased. A subtle reaction in cochlear function was found in neonates after exposure to 3.0T MRI noise with hearing protection.