Renal fibrosis is a hallmark of chronic kidney disease, which drives further kidney injury and leads to renal failure. It is critical to assess the spatiotemporal extent of fibrosis in kidney. However, currently there are no reliable non-invasive means for assessing the severity and progression of fibrosis in individual kidney. Therefore, here we evaluate the utility of MT imaging for measuring renal fibrosis in kidney using hHB-EGFTg/Tg mouse model, in which progressive fibrosis is the predominant event. In this study, we compare and assess the utility of MTR and PSR for detecting tubulointerstitial fibrosis in OSOM of kidney.