Glomerular fibrosis develops in multiple types of chronic kidney diseases. This study aims to explore the promising MRI methods for quantifying glomerular fibrosis, and to evaluate the feasibility of performing the measurements for rat kidneys using clinical MRI scanner. Four healthy and 4 fibrotic rats were included in the experiment. With fibrosis, significant changes were found in cortical and medullary T1 and T2. Measurement of T2* suffered from susceptibility artifact. This study verified that clinical MRI scanners can be used to monitor the development of renal fibrosis in rat kidneys.