We investigated the metabolic effect of a clinically relevant craniospinal irradiation on the rat spinal cord using 1H MRS at 7T. Hundred days after the fractionated irradiation performed by radioactive isotope 60Co (16Gy in 2 fractions), irradiated and sham-irradiated animals underwent in vivo 1H MRS examination. Spinal cord spectra were measured by SVS sequence with voxel size of 3.5x2x7.5mm3. In spinal cord of irradiated animals was confirmed significantly reduced tNAA/tCr and increased tCho/tCr ratio, both showing demyelination and inflammatory processes. It seems that in vivo 1H MRS of spinal cord could be useful for radiation therapy monitoring.