31P measurement of the oxidized (NAD+) and reduce form (NADH) of nicotinamide adenine dinucleotide has been applied to in vivo assessment of the redox state of the brain. Since uridine diphosphate glucose (UDPG) overlaps with the nicotinamide adenine dinucleotide peaks we examined the effect of changes in the relative intensities of the UDPG basis components on quantification of the redox ratio. We found that the fitted redox ratio is significantly dependent on the UDPG basis whose chemically distinct components are subject to different T1 saturation effect in vivo.
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