Abstract #3121

Mapping astroglial glutamine synthetase activity in vivo in a preclinical model of epilepsy using glutamate-weighted CEST (GluCEST) MRI

Puneet Bagga¹, Stephen Pickup¹, Julien Flament², John Detre³, Hari Hariharan¹, and Ravinder Reddy¹

¹Department of Radiology, University of Pennsylvania, Philadelphia, PA, United States, ²Molecular Imaging Research Center (MIRCen), Paris, France, ³Department of Neurology, University of Pennsylvania, Philadelphia, PA, United States

Epilepsy is broadly characterized by aberrant neuronal excitability causing seizures. Glutamate is the major excitatory neurotransmitter in the brain and can be detected using MRS and glutamate weighted chemical exchange saturation transfer (GluCEST) MRI. In this study, we performed high-resolution GluCEST MRI in a preclinical model of epilepsy to evaluate glutamatergic alterations in the hippocampus. GluCEST contrast was increased in the hippocampus of mice treated with methionine sulfoximine acutely, while mice treated chronically with MSO showed reduced hippocampal GluCEST contrast. The GluCEST results were also corroborated with in vivo ¹H MRS.

How to access this content:

For one year after publication, abstracts and videos are only open to registrants of this annual meeting. Registrants should use their existing login information. Non-registrant access can be purchased via the ISMRM E-Library.

After one year, current ISMRM & ISMRT members get free access to both the abstracts and videos. Non-members and non-registrants must purchase access via the ISMRM E-Library.

After two years, the meeting proceedings (abstracts) are opened to the public and require no login information. Videos remain behind password for access by members, registrants and E-Library customers.

Click here for more information on becoming a member.